![]() ![]() This combined workflow enables contiguous Linked-Reads and maximal long-range phasing information.Īccess new regions of the genome, uniquely called structural variants, and phased compound heterozygotes with the Chromium™ Exome Solution. ![]() SureSelect phased exome baits are used to isolate selected content for exome sequencing and fill gaps while targeting heterozygous SNPs. The Chromium Exome Solution massively partitions and barcodes DNA using microfluidics, producing sequencing-ready libraries with >1,000,000 unique barcodes. The Chromium Exome Solution utilizes molecular barcoding of long DNA molecules, updated capture probes, and improved informatics to produce the most complete exome assay. Start with only 1 ng high molecular weight DNA (HMW gDNA)Įxome sequencing identifies the causal variant for roughly 25-50% of individuals assessed, suggesting the need for improved methods.Turn-key analysis pipeline and visualization tools.The Fragment Analyzer allows for high throughput nucleic acid fragment analysis. 10X Chromium Single Cell solutions can capture between 500 10,000 cells from samples with cell concentrations of 100 2000 cells/uL. Each chip can process 8 samples at once, capturing 80,000 in less than 20 minutes. Detect both balanced and unbalanced large structural variants The 10X Chromium Controller generates thousands of single cell nano-reactions to barcode up to 10,000 cells per lane for high throughput single cell analysis.Access new genomic content with 10x molecular barcode enhanced alignment.Phase the genome and generate the full spectrum of high quality variants. ![]() Uncover new regions of the genome with the Chromium™ Genome Solution. Technologies in the STU 10x Genomics Visium 10x Genomics Chromium Connect Akoya Polaris Vectra Akoya CODEX Bruker Vutara VXL NanoString GeoMx DSP Vizgen. The Chromium Software Suite, which includes the Long Ranger™ software package and the Lariat™ Aligner, provides turn-key analysis pipelines and visualization tools utilizing Linked-Read data. Final libraries are compatible for sequencing on most Illumina systems, including the HiSeq X. After molecular barcoding, fragments are pooled and undergo standard library preparation. Powered by the GemCode™Technology, the Chromium™ Genome Solution massively partitions and molecularly barcodes DNA using microfluidics, producing sequencing-ready libraries with >1,000,000 unique barcodes. Here, we develop a method that uses DNA probes and leverages an existing commercial microfluidic platform (10X Chromium) to conduct bacterial single cell RNA sequencing. The Chromium Genome Solution uses the power of Linked-Reads to fully resolve genic phasing, structural variation, and detect variants in previously inaccessible and complex regions of the genome. ![]()
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